Non-mechanically-axial-scanning confocal microscope using adaptive mirror switching

Yoshiaki Yasuno, Shuichi Makita, Toyohiko Yatagai, Tobias Wiesendanger, Aiko Ruprecht, Hans Tiziani
2003 Optics Express  
A non-axial-scanning confocal microscope employing a monochromatic light source has been developed. The system controls the defocus of an objective into three to five optimized states by using a membrane-adaptive mirror, and determines the axial height of an object according to the confocal output value with each defocus. A genetic algorithm is employed to optimize the adaptive mirror shape, with the information entropy of the spectrum of the lateral confocal spot profile used as a cost
more » ... d as a cost function in the genetic algorithm. Our experimental system successfully determined axial object height within 50 µm range with 0.64 % of error.
doi:10.1364/oe.11.000054 pmid:19461705 fatcat:z4qhv27ytbhx5ijnn3xnxxio7m