Diagnosis ofTrypanosomaevansibythepolymerase chainreactioTl(PCR) J.E,DONELSONland W.T,ARTAMA2 1〃叩佃J川J可、飢′(ソ′〃〃f、-帆仙れ,ピr-巾げノり叫ん肌∫(■叫ノ棚 ∫5224ヱ....九州イコJU( 月わJど(イけ-〝J〃gγ,U〝ル肌巾〝(加¢〟仙加ゎ,〃.乃たJ此(JUJ(肌一,肋血=旬Ⅴ(血〃仇J〃〃〃〃〃.一丁A 王ミじい、iledコ()A哩uゝ1 †早け Keヽ′Ⅵ′()ー山:丁ハp〝JJ... (一川〟ぞ叩几−fこdjagnいSisこP()l〉rmeraSeChainre鉱山on A艮STRACT Tbpan〃LW,maeVanLYiandthethreesubspeCiesof7二bruceiareindistinguishablefrom eachothermorphoIoglCallyandarethoughttobeverycloselyrelatedevolutionari]y,The mos(notable moleculardifference

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... n r.evansiand the TIJ,r〟Ceisubspeciesis the StruCtureOftheDNA molecu]esfoundintheir kinetoplast.asubceLlularorganellelocated nearthe flagellar pocket,We have exploited this differencein kDNA sequence and Organizationtodeve]opapolymerasechainreaction(PCR)−basedassay thatcandistinguis betweenT.evansiandTlbr〟Cei.0】igonucleotideprlmerSSpaCedabout370bpapartonthe T.evan5ikDNAsequencegenerateaPCR−amplificationproductwhentota17二elJa血DNA isusedasthetemplate,両IereaSnOamp】ificationproductoccurs whentotaJDNAfrom Tl br〟Ceior LeisFmuniais the template.Jn contrast、WhenⅨ二R primers speCific for three differentnuclearDNAsequencesofT・bTuCei−a177−bprepeat,theprocyclinge11eOrthe SPlicedleader gene−are uSedin similarⅨ二Ramplifications,identica]PCRproducts are O♭tainedfromtheT.br〟CeiandTevansitcmplatcDNAs・Thus,thenuclearDNAsequences OfT.evansiandT.bruceiareveryshTlilar,buttheirkDNAsaresufncientJydifferenttoserve asthebasisforPCR−baseddiagnosisof(hetwotrypanOSOmeSpeC)eS−