Analysis of mRNA-lncRNA and mRNA-lncRNA-Pathway co-expression networks based on WGCNA in developing pediatric sepsis
Background: Pediatric sepsis is a great threat in death worldwide. However, the pathogenesis has not been clearly understood until now in sepsis.Methods: This study identified differentially expressed mRNA (DEMs) and lncRNAs (DELs) based on Gene Expression Omnibus (GEO) database. And the weighted gene co-expression network analysis (WGCNA) was performed to explore co-expression modules associated with pediatric sepsis. Then Gene Ontology (GO), KEGG (Kyoto Encyclopedia of Genes and Genomes)
... s and Genomes) pathway, DEMs‑DELs and DEMs‑DELs-Pathway co-expression network analysis was conducted in selected significant module. Results: A total of 1941 DEMs and 225 DELs were used to conduct WGCNA. And the turquoise module was selected as the significant module that was associated with particular traits. The DEMs functions associated with many vital processes were also shown by GO and KEGG pathway analysis in the turquoise module. Finally, 15 DEMs and 4 DELs (GSEC, NONHSAT160878.1, XR_926068.1 and RARA-AS1) were selected as candidate biomarkers in DEMs-DELs-Pathway co-expression network. Conclusions: Our study identified 15 DEMs and 4 DELs as diagnostic markers, which could also provide more directions to study molecular mechanism of pediatric sepsis.