Th is study reports, for the fi rst time, an effi cient in vitro plant regeneration protocol for Digitalis lamarckii Ivan. (dwarf foxglove) via direct shoot organogenesis. Two sets of experiments were carried out; the fi rst compared diff erent concentrations of 6-benzylaminopurine (BAP), kinetin, thidiazuron (TDZ), and zeatin alone using leaf explants excised from in vitro germinated seedlings, while the second set tested the combinations of indole-3-butyric acid (IBA) with BAP, kinetin, TDZ,

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... nd zeatin for shoot multiplication from the leaf explants, which were already cultured and developed numerous shoots during the fi rst set of experiments. For shoot regeneration (the fi rst set of experiments), TDZ was the most eff ective at 1.0 mg L -1 concentration, producing a mean of 10.3 shoots per explant and was signifi cantly more eff ective than BAP. For shoot multiplication (the second set of experiments), a combination of 0.2 mg L -1 IBA with 0.2 mg L -1 TDZ produced signifi cantly more shoots per explant (16.5 shoots) than with BAP (11.0 shoots), zeatin (5.5 shoots), or kinetin (4.0 shoots). Th e regenerated shoots were readily rooted on medium containing 0.5 mg L -1 indole-3-acetic acid (IAA). Rooted regenerants were then transferred to the pots, where they grew well and attained maturity. Th e described protocol provides a simple way to regenerate plants through direct shoot organogenesis, which would be useful for a large-scale production of cardenolides, germplasm conservation, and genetic transformation studies in this medicinally important endemic species. Endemik tıbbi bir tür olan Digitalis lamarckii'nin yaprak eksplantlarından direkt sürgün rejenerasyonu Özet: Digitalis lamarckii (bodur yüksükotu) için direkt sürgün organogenezisi yolu ile etkili bir in vitro rejenerasyon protokolu ilk defa bu çalışma ile rapor edilmektedir. İki farklı deney seti kurulmuştur; ilk sette, in vitro koşullarda çimlendirilmiş fi delerden alınan yaprak eksplantları kullanılarak, 6-benzilaminopürin (BAP), kinetin, thidiazuron (TDZ) ve zeatinin farklı konsantrasyonları karşılaştırılmış, ikinci sette ise sürgün çoğaltımı amacıyla, birinci sette kültüre alınan ve çok sayıda sürgün meydana getirmiş olan eksplantlar kullanılarak, indol-3-butirik asidin (IBA), BAP, kinetin, TDZ ve zeatin ile kombinasyonları test edilmiştir. Sürgün rejenerasyonu (birinci deney seti) sonuçlarına bakıldığında, TDZ'nin, BAP' den istatistiki olarak çok daha etkili olduğu bulunmuş, 1,0 mg L -1 dozunda kullanıldığında ise eksplant başına ortalama 10,3 sürgün üreterek, en başarılı sonucu vermiştir. Sürgün çoğaltımı (ikinci deney seti)