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Genetic Transformation in Crops [Working Title]
With easier access to genome sequences and genome editing technology over the last few years, transformation technologies are routinely being used now as a research tool for elucidating gene function. In this chapter, we outline a simple A. tumefaciens-mediated transformation method for the diploid brassica species B. oleracea, using a doubled haploid spring line called AG DH1012. This genotype has become our model genotype for training purposes and for most of our routine work, with adoi:10.5772/intechopen.93570 fatcat:33bhbd7xfnblxf2xgk6vsdyzt4