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The projection of GnRH neurons to the median eminence of the medial basal hypothalamus (MBH) is established early in development and is also seen when preoptic area-derived GnRH cell-containing grafts are placed in the third ventricle of hypogonadal mice. To further study the factors directing GnRH axonal targeting, we cultivated embryonic or postnatal day 1 preoptic area with a coexplant on collagen-and laminin-coated membranes in insert chambers. After 7 days of culture, GnRH-immunoreactivedoi:10.1210/en.138.9.3956 pmid:9275087 fatcat:4pxiunoiare5lm3qlzguena7ee