Two-dimensional difference gel electrophoresis applied for analytical proteomics: fundamentals and applications to the study of plant proteomics

Sandra Cristina Capaldi Arruda, Herbert de Sousa Barbosa, Ricardo Antunes Azevedo, Marco Aurélio Zezzi Arruda
2011 The Analyst  
The present review reports the principles, fundamentals and some applications of two-dimensional difference gel electrophoresis for analytical proteomics based on plant proteome analysis, also emphasizing some advantages of 2-D DIGE over 2-D PAGE techniques. Some fluorescent protein labeling reagents, methods of protein labeling, models of 2-D DIGE experiments, and some limitations of this technique are presented and discussed in terms of 2-D DIGE plant proteomes. Finally, some practical
more » ... tions of this technique are pointed out, emphasizing its potentialities in plant proteomics.
doi:10.1039/c1an15513j pmid:21879037 fatcat:vhrgymhi3rbbzgceub6i4k3hve