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Burul ulcer disease is an infection of the subcutaneous layer. The causative organism, Mycobacterium ulcerans is a slow-growing environmental pathogen often associated with wetland and slow moving streams. One insect order believed to be associated with M.ulcerans is the order hemiptera, in which the aquatic bugs (Naucoris sp and Belostoma sp) belong. Some Mycobacteria species are endosymbiont of Acanthamoeba and laboratory experiments has confirmed this in M. ulcerans in an endemic area indoi:10.7287/peerj.preprints.656v2 fatcat:ijdefrt63zggxbrkm6ynnfwski