Identification and purification of a protein encoded by the human adenovirus type 2 transforming region

M Green, K H Brackmann, M A Cartas, T Matsuo
1982 Journal of Virology  
The human adenovirus type 2 (Ad2) transforming genes are located in early regions Ela (map position 1.3 to 4.5) and Elb (map position 4.6 to 11.2). We have identified and purified to near homogeneity a major 20,000-molecular-weight (20K) protein and have shown that it is coded by Elb. Using an Ad2-transformed cell antiserum which contained antibody to Elb-coded proteins, we immunoprecipitated 53K and 19K proteins from the nucleoplasm and 53K, 19K, and 20K proteins from the cytoplasmic S-100
more » ... tion of Ad2 productively infected and Ad2-transformed cells. The 19K protein was present in both the nucleoplasm and the cytoplasm, whereas the 20K protein was found only in the cytoplasm. The 53K and 19K proteins are known Ad2 Elb-coded proteins. The 20K protein was on May 9, 2020 by guest
doi:10.1128/jvi.42.1.30-41.1982 fatcat:qpyc6b2jejeqvo3lwgvns74aga