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The gut microbiome and the gut brain axis are potential determinants of Alzheimer's disease (AD) etiology or severity and gut microbiota might coordinate with the gutbrain axis to regulate behavioral phenotypes in AD mouse models. Using 6-month-old human amyloid precursor protein (hAPP) knock-in (KI) mice, which contain the Swedish and Iberian mutations [APP NL-F (App NL−F )] or the Arctic mutation as third mutation [APP NL-G-F (App NL−G−F )], behavioral and cognitive performance is associated
more » ... ith the gut microbiome and APP genotype modulates this association. In this study, we determined the feasibility of behavioral testing of mice in a biosafety cabinet and whether stool from 6-month-old App NL−G−F mice or App NL−G−F crossed with human apoE4 targeted replacement mice is sufficient to induce behavioral phenotypes in 4-5 monthold germ-free C57BL/6J mice 4 weeks following inoculation. We also compared the behavioral phenotypes of the recipient mice with that of the donor mice. Finally, we assessed cortical Aβ levels and analyzed the gut microbiome in the recipient mice. These results show that it is feasible to behaviorally test germ-free mice inside a biosafety cabinet. However, the host genotype was critical in modulating the pattern of induced behavioral phenotypes as compared to those seen in the genotype-and sexmatch donor mice. Male mice that received stool from App NL−G−F and App NL−G−F/E4 donor genotypes tended to have lower body weight as compared to wild type, an effect not observed among donor mice. Additionally, App NL−G−F/E4 recipient males, but not females, showed impaired object recognition. Insoluble Aβ40 levels were detected in App NL−G−F and App NL−G−F/E4 recipient mice. Recipients of App NL−G−F, but not App NL−G−F/E4 , donor mice carried cortical insoluble Aβ40 levels that positively correlated with activity levels on the first and second day of open field testing. For recipient mice, the interaction between donor genotype and several behavioral scores predicted gut microbiome alpha-diversity. Similarly, two behavioral performance
doi:10.3389/fnbeh.2022.791128.s001 fatcat:3ulurlzpwrgrha3jcjcdn4dpry