The Phylogeny of Prokaryotes Associated with Australia's Great Basin

Mark Spanevello, Bharat Patel, University, My
2019
The Great Artesian Basin of Australia is the largest freshwater artesian basin in the world. It underlies arid and semi-arid regions of Queensland, New south Wales, South Australia and the Northern territory, approximately 20% of the Australian continent. Temperatures of its' waters range from 30°C to over 100°C and over 5000 bores access its waters and empty into open drainage systems for agricultural irrigation and stock watering purposes. The Great Artesian Basin of Australia has great
more » ... lia has great economic and agricultural importance, but prokaryotic communities persisting in the bore waters influence its' quality. To gain an understanding of these prokaryotic communities, a culture-independent study was conducted on microbial communities present in the outflow of the New Lorne bore (registered bore number 17263). Five distinct prokaryotic communities existing at different temperatures (88°C, 75°C, 66°C, 57°C, and 52°C) were selected and total genomic DNA was extracted. PCR-amplified 16S rRNA genes were subsequently cloned and a total of 64 clones from the 88°C community and 96 clones from the other communities were examined. Partial sequences identified phylotypes that were then fully sequenced and analysed phylogenetically. The analysis revealed identical phylotypes existing in adjacent communities, as well as an increase in the phylogenetic diversity as water temperature decreased. Sequences identified belonged to species spanning the full diversity of the Bacterial domain, including Hydrogenobacter, Thermus, Meiothermus, Chloroflexus, Cytophaga, Planctomycetes, Rhodothermus, Bacillus, Clostridium, Nitrospira, Verrucomicrobium, Acidobacterium, α-, β-, γ-, and δ-Proteobacteria. Clones recovered also grouped with taxa with no isolated representatives. Of the libraries, 24 phylotypes from 6 phyla had a similarity of 96% or more to cultured isolates and comprised 73% of all clones analysed. 34 phylotypes from 11 phyla had less than 96% similarity to cultured isolates, or were related to previously cloned 16S rRNA gene [...]
doi:10.25904/1912/3859 fatcat:yxaikam72fhotmq7wun43jghia