Spectroscopic Evidence That Salicylic Acid Converts a Temporally Inactivated Form of Horseradish Peroxidase (Compound III) to the Irreversibly Inactivated Verdohemoprotein (P-670)

Tomonori KAWANO, Shoshi MUTO, Masaru ADACHI, Hiroshi HOSOYA, Frédéric LAPEYRIE
2002 Bioscience, biotechnology and biochemistry  
We obtained spectroscopic evidence in support of salicylate-dependent inactivation of horseradish peroxidase-C. Addition of salicylate to the enzyme arrested at a temporal inactive state (Compound III) in the presence of H 2 O 2 , resulted in rapid and irreversible inactivation of the enzyme yielding verdohemoproteins (P-670). Multiple roles for salicylate in peroxidasecatalyzed reactions are discussed.
doi:10.1271/bbb.66.646 pmid:12005063 fatcat:blckboxlezh23dce7r37ddx7mq