Inhibitory effect of O-glycosylation inhibition on human intestinal epithelial cells Mucin 2 expression and bacteria adherence

Li-li SONG, Jun YE, Yun LIU, Qiong PAN, Xiao-li ZHONG, Shan-shan LI, Yin TIAN, Zhi-hong PENG, Rong-quan WANG
2013 Medical Journal of Chinese People's Liberation Army  
Objective To investigate the effect of O-glycosylation inhibition in intestinal epithelial cells on the expression of Mucin 2 (MUC2) and bacterial adherence. Methods Intestinal epithelial cells HT-29 and differentiated HT-29 cells (HT-29-Gal) were treated with an inhibitor of O-glycosylation (benzyl-α-GalNAc), and then named as HT-29-OBN and HT-29-Gal-OBN, respectively. The mRNA and protein expression of MUC2 in HT-29, HT29-Gal, HT-29-OBN and HT-29-Gal-OBN were detected by real-time PCR and
more » ... ern blotting. Then the four kinds of above cells were incubated with enteropathogenic Escherichia coli (EPEC) or enterohemorrhagic Escherichia coli serotype O157:H7 (EHEC O157:H7). The bacteria were quantified by determining the colony forming unit (CFU) following the plating of serial dilutions of the bacteria to evaluate the effect of benzyl-α-GalNAc on bacteria adherence. Results The results of real-time PCR and Western blotting showed that the mRNA and protein expression levels of MUC2 in HT-29-OBN and HT-29-Gal-OBN cells were significantly lower than those in the untreated cells HT-29 and HT-29-Gal (P<0.05). The bacterial adherence assay showed that the adherence of EPEC and EHEC O157:H7 to HT-29-OBN and HT-29-Gal-OBN cells significantly decreased compared with that to HT-29 and HT-29-Gal cells (P<0.05). Conclusion Inhibition of O-glycosylation in intestinal epithelial cells may reduce the bacteria adherence and MUC2 expression. DOI: 10.11855/j.issn.0577-7402.2013.10.009
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