Ruscogenin Mainly Inhibits Nuclear Factor-κB but Not Akt and Mitogen-Activated Protein Kinase Signaling Pathways in Human Umbilical Vein Endothelial Cells

Jiaxi Song, Junping Kou, Yalin Huang, Boyang Yu
2010 Journal of Pharmacological Sciences  
Our previous results suggested that ruscogenin inhibited tumor necrosis factor α (TNF-α)-induced leukocyte adhesion, which correlated with its suppression of intercellular adhesion molecule-1 (ICAM-1) expression in endothelial cells. In the present studies, we further examined its effects on the main signaling pathways involved in upregulation of ICAM-1 induced by TNF-α in human umbilical vein endothelial cells (HUVECs). The results showed that ruscogenin significantly suppressed p65
more » ... tion, IκB-α phosphorylation and degradation, and inhibited IκB kinase α (IKKα) and IKKβ activation induced by TNF-α. However, it exerted weak effects on TNF-α-induced phosphorylations of p38, JNK, ERK1/2, and Akt. Overall, our results indicated that downregulation of ICAM-1 expression by ruscogenin in HUVECs might be mediated by nuclear factor-κB (NF-κB), but not by mitogen-activated protein kinase (MAPK) and Akt signaling pathways.
doi:10.1254/jphs.10076sc pmid:20724805 fatcat:w3rnapiss5a45puya2gr6n5jnm