Glycocalyx-mediated Cell Adhesion and Migration [article]

Samuel Schmidt, Bettina Weigelin, Joost te Riet, Veronika te Boekhorst, Mariska te Lindert, Mietske Wijers-Rouw, Barbara Lelli, Lorenz Rognoni, Marina Krause-Vortmeyer, Anthea Messent, Luisa Bracci, Kay Eberhard Gottschalk (+5 others)
2020 bioRxiv   pre-print
Cell migration is a force-dependent adaptive process mediated by integrin-dependent adhesion as well as other yet poorly defined interactions to the extracellular matrix. Using enzymatic multi-targeted digestion of sugar moieties on the surface of mesenchymal cells and leukocytes after interference with integrin function, we demonstrate that the surface glycocalyx represents an independent adhesion system. The glycocalyx mediates cell attachment to ECM ligand in the 100-500 pN force range and
more » ... oeboid migration in 3D environments in vitro and in vivo. Glycan-based adhesions consist of actin-rich membrane deformations and appositions associated with bleb-like and other protrusions forming complex-shaped sub-micron contact sites to ECM fibrils. These data implicate the glycocalyx in mediating generic stickiness to support nanoscale interactions (nanogrips) between the cell surface and ECM, mechano-coupling, and migration.
doi:10.1101/2020.06.12.149096 fatcat:cg7p2pgigrabxacxlewre2pdwy