High-mannose oligosaccharides from human Tamm-Horsfall glycoprotein

Franca Serafini-Cessi, Fabio Dall'olio, Nadia Malagolini
1984 Bioscience Reports  
The present communication reports the occurrence of high-mannose oligosaccharides on Tamm-Horsfatl glycoprotein prepared from human pooled urine. The Pronase digest of the glycoprotein was fractionated by gel filtration and a high-mannose glycopeptide species was separated from complex-type glycopeptides. When high-mannose glycopeptides were digested with endo-13-N-acetylglucosaminidase H, followed by reduction with [SHJKBH~, three oligosaccharides were resolved by thin-layer chromatography. On
more » ... the basis of chromatographic mobility and exoglycosidase digestions the composition Man7-, ManE-, and Mans-GlcNAc was assigned to the three oligosaccharides. Man6GlcNAc is by far the major component.
doi:10.1007/bf01119663 pmid:6722291 fatcat:25hfafbl55bkznkm6zss5er74u