Immobilization of Phytase from Rice Bean (Vigna umbellata Thunb.) on Glutaraldehyde Activated Chitosan Microspheres

K. Belho, P. K. Ambasht
2021 Journal of scientific research  
The immobilization of phytase from rice bean was optimized on the glutaraldehyde-activated chitosan microsphere, and characterized. The optimum percent immobilization was 77.48%, when microspheres were prepared with 1.5% chitosan, activated with glutaraldehyde (0.5%, for 4 h) and with the enzyme protein (0.2 mg/mL, and coupling time 4 h). The immobilized phytase exhibited elongated fibres and pores. FTIR results suggest the formation of a Schiff base with a band at 1638 cm -1 . The pH and
more » ... . The pH and temperature optimum of immobilized phytase was 4.0 and 40 °C, respectively. The energy of activation was 34.5 kJ/mol. The Km and Vmax values of immobilized phytase were 0.62 mM and 3.42 μmol/min. The immobilized enzyme when incubated at 50 °C, retained 59% activity after 75 min. The immobilized phytase showed 80% activity retention, after 14 days, when stored at 4 °C. The immobilized phytase could be reused for 4 cycles with 58% activity retention. The immobilized phytase, when incubated for 60 min in the presence trypsin and pepsin, showed 92.9 and 95% activity retention, respectively. The properties of immobilized phytase did not alter with respect to pH, and temperature optima. Immobilized phytase exhibited proteolytic resistance when incubated with pepsin for 1 h, and this can find application in animal feed.
doi:10.37398/jsr.2021.650115 fatcat:3bkp3p4eb5ey7mvuiccuaayuvm