A cytofluorimetric analysis of a Saccharomyces cerevisiae population cultured in a fed-batch bioreactor

Emanuela Palomba, Valentina Tirelli, Elisabetta de Alteriis, Palma Parascandola, Carmine Landi, Stefano Mazzoleni, Massimo Sanchez, Alvaro Galli
2021 PLoS ONE  
The yeast Saccharomyces cerevisiae is a reference model system and one of the widely used microorganisms in many biotechnological processes. In industrial yeast applications, combined strategies aim to maximize biomass/product yield, with the fed-batch culture being one of the most frequently used. Flow cytometry (FCM) is widely applied in biotechnological processes and represents a key methodology to monitor cell population dynamics. We propose here an application of FCM in the analysis of
more » ... the analysis of yeast cell cycle along the time course of a typical S. cerevisiae fed-batch culture. We used two different dyes, SYTOX Green and SYBR Green, with the aim to better define each stage of cell cycle during S. cerevisiae fed-batch culture. The results provide novel insights in the use of FCM cell cycle analysis for the real-time monitoring of S. cerevisiae bioprocesses.
doi:10.1371/journal.pone.0248382 pmid:34111115 fatcat:omoy2x45mnfojnbypxx3pkv6t4