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The aim of this study was to develop HPLC method capable of facilitating the simultaneous determination of ciprofloxacin hydrochloride (CIP.HCl) and metronidazole (MDZ). The analytes were separated with Lichrospher 100 RP-18 C18 column (100 x 4.6 mm, 5 μm). The mobile phase consisted of monobasic potassium phosphate (50 mM, pH 3.5) and acetonitrile (80: 20, v/v) containing triethylamine (7.5 mM) delivered isocratically with flow rate of 1.0 mL/min. The UV detection was set 298 nm. The developeddoi:10.11113/jt.v72.3071 fatcat:h65jquf5mbdhlfpufyfq2gfdam