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Microfluidic construction of minimalistic neuronal co-cultures
Lab on a Chip
In this paper we present compartmentalized neuron arraying (CNA) microfluidic circuits for the preparation of neuronal networks using minimal cellular inputs (10-100-fold less than existing systems). The approach combines the benefits of microfluidics for precision single cell handling with biomaterial patterning for the long term maintenance of neuronal arrangements. A differential flow principle was used for cell metering and loading along linear arrays. An innovative water masking techniquedoi:10.1039/c3lc41224e pmid:23403713 fatcat:ltgjlaza3jcorgd3l3ebbwngbq