The aim of this study was to investigate the effect of growth hormone on biomass and production of bilobalide and ginkgolides A and B bycallus and cell suspension cultures of Ginkgo bilobaleaves. Calluses were induced from Ginkgo biloba leaves on Murashige and Skoog (MS) medium supplemented with sucrose and combination of growth hormone in several concentrations. The production of bilobalide, ginkgolides A and B in callus with combination of 2 mg/L NAA and 0.1 mg/L kinetin showed the highest.

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... enhance the biomass and production of bilobalide, ginkgolides A and B, effects of growth hormone, NO 3 -, and carbon source were investigated in cell suspension cultures. Among several concentrations of kinetin + NAA and kinetin + 2,4-D, the highest content of bilobalide, ginkgolides A and B was observed in MS medium supplemented with 0.1 mg/L kinetin + 2 mg/L NAA. The production of bilobalide, ginkgolides A and B in the addition of glucose was higher than that of sucrose and fructose. Reducing 50% of KNO 3 in the medium decreased the biomass and the content of bilobalide, ginkgolides A and B.