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Aims-To evaluate the usefulness of a single-tube nested polymerase chain reaction (PCR) assay in the diagnosis of tuberculosis in 1497 pulmonary and 536 extrapulmonary specimens. Methods-A single-tube nested PCR, utilising two sets of primers with different melting temperatures (88'C for external primers; 70°C for internal primers) to augment sensitivity and specificity without increasing the risk of amplicon contamination, was evaluated. Specimens were initially tested for the repetitivedoi:10.1136/jcp.49.4.290 pmid:8655703 pmcid:PMC500452 fatcat:njytqodkzreufdozpkz7kejmwu