Optimization of the asymmetric synthesis of chiral aromatic alcohol using freeze-dried carrots as whole-cell biocatalysts

Hilal Celik Kazici, Emine Bayraktar, Ülkü Mehmetoglu
2016 Green Processing and Synthesis  
AbstractAsymmetric reduction of ketones is an important transformation in organic synthesis, because chiral carbinols are useful bioactive compounds. In this study, bioreduction of acetophenone (ACP) for production of enantiomerically pure (S)-1-phenyl-ethanol was investigated and freeze-dried carrots were used as a source of alcohol dehydrogenases (ADHs). However, production of product was investigated systematically using response surface methodology (RSM). Before RSM, the effects of the
more » ... al substrate concentration, reaction time, temperature and pH on the bioreduction were studied. The best results for enantiomeric excesses (ee) and conversion (c) were obtained with >99% and 58%, respectively, for the reaction time 48 h, initial substrate concentration 1 m
doi:10.1515/gps-2015-0118 fatcat:tatbv6gw6rbgxkkhlh6pwkhcji