P15.06 Molecular validation of putative antimicrobial peptides for improved human immunodeficiency virus diagnostics via hiv protein p24
Sexually Transmitted Infections
activation and inflammation in the genital tracts of adolescents from South Africa. Methods Cervical cytobrush mononuclear cells were isolated from 148 adolescents (16-22 years) from Cape Town, and expression of T-cell activation and proliferation markers (CD38, HLA-DR, Ki67, CCR5) was measured by FACs. Adolescents were screened for BV (Nugent) and STIs (C. trachomatis, N. gonnorhoea, T. vaginalis, M. genitalium, HSV-2) by PCR. For comparison, 11 HIV-negative adult women were included.
... included. Concentrations of 48 cytokines, chemokines and growth factors were measured in matching menstrual cups by Luminex. Results Adolescents (median 18 years; IQR 17-20) had significantly higher frequencies of activated CD4 + T-cells (CD38 + , HLADR + , CD38 + HLADR + : each p < 0.0001) from cervical cytobrushes than adults although CCR5 expression was higher in adults. STIs and BV prevalence was very high, with 71% of adolescents having ‡1 STI and/or BV, and 42% being C. trachomatis positive. Adolescents with an STI, despite these being asymptomatic, had higher frequencies of activated and proliferating T-cells compared to those with no STI/BV (CD4 + CD38 + HLADR + : p = 0.047; CD4 + Ki67 + : p = 0.020). Women positive for chlamydia had significantly higher frequencies of CD4 + CD38 + T-cells (p = 0.006). Women with both STIs and BV had the most pronounced increase in CD4 + T-cell activation (CD38 + : p = 0.002; CD38 + HLADR + : p = 0.001; Ki67+: p = 0.002). Higher cervical T-cell activation marker expression was directly associated with increased genital cytokine profiles. Conclusion Heightened levels of genital immune activation and inflammation found in South African adolescent females, partly due to the presence of asymptomatic STIs and BV could increase their risk for HIV infection.