MANNER OF ATTACHMENT OF FLAGELLA IN VIBRIO COMMA

Jutaro Tawara
1964 Journal of Bacteriology  
The manner in which the flagella are attached on Vibrio comma was reported previously (Tawara, J. Bacteriol. 73:89, 1957). It was then demonstrated that each flagellum was attached to a granule embedded in the cytoplasm of V. comma. Recently, the negative-staining technique has come to be used extensively in electron microscopic study, and this method was employed in the present observation of the flagella. As a result, a somewhat different picture was obtained as compared with previous
more » ... th previous investigation. For this observation V. comma Inaba strain was cultured for 36 hr on nutrient agar adjusted o pH 7.8. The bacteria were negatively stained with 1 % phosphotungstic acid before electron microscopic observation. Figure 1 shows an electron micrograph of the vibrio stained with phosphotungstic acid for 10 min. It can be seen that a flagellum pierces the cell wall and is attached to a granule in the cytoplasm. This granule gives the appearance of being surrounded by a thin layer of poorly stained cytoplasm. Figure 2 is an electron micrograph of a chromium-shadowed specimen showing a similar granule. In this instance, the granule appears structureless. It is concluded that in V. comma the flagella and the granules in the cytoplasm are attached in the manner of a cherry pit to its stem. Frf F
doi:10.1128/jb.88.2.531-532.1964 fatcat:qn4j2uqs6ba5nnkn5cirshcgia