Jutaro Tawara
1964 Journal of Bacteriology  
The manner in which the flagella are attached on Vibrio comma was reported previously (Tawara, J. Bacteriol. 73:89, 1957). It was then demonstrated that each flagellum was attached to a granule embedded in the cytoplasm of V. comma. Recently, the negative-staining technique has come to be used extensively in electron microscopic study, and this method was employed in the present observation of the flagella. As a result, a somewhat different picture was obtained as compared with previous
more » ... th previous investigation. For this observation V. comma Inaba strain was cultured for 36 hr on nutrient agar adjusted o pH 7.8. The bacteria were negatively stained with 1 % phosphotungstic acid before electron microscopic observation. Figure 1 shows an electron micrograph of the vibrio stained with phosphotungstic acid for 10 min. It can be seen that a flagellum pierces the cell wall and is attached to a granule in the cytoplasm. This granule gives the appearance of being surrounded by a thin layer of poorly stained cytoplasm. Figure 2 is an electron micrograph of a chromium-shadowed specimen showing a similar granule. In this instance, the granule appears structureless. It is concluded that in V. comma the flagella and the granules in the cytoplasm are attached in the manner of a cherry pit to its stem. Frf F
doi:10.1128/jb.88.2.531-532.1964 fatcat:qn4j2uqs6ba5nnkn5cirshcgia