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A 978-base-pair gene that encodes thymidylate synthase (TS; 5,10-methylenetetrahydrofolate:dUMP Cmethyltransferase, EC 188.8.131.52) from Lactobacillus casei has been synthesized and inserted into Escherichia coli expression vectors. The DNA sequence contains 35 unique restriction sites that are located an average of 28 base pairs apart throughout the entire length of the gene. A ribosome binding site was included 9 base pairs upstream from the translation start site and codon usage was adjusted todoi:10.1073/pnas.87.2.633 pmid:2153960 pmcid:PMC53319 fatcat:z56lwtrbg5es3e5yhqq2bivpiu