In Vitro Cytotoxic Activity of Two Malaysian Rainforest Plants on Colon Carcinoma Cell-Line Keywords: *Corresponding author: MATERIALS Plant material Preparation of extracts Cell culture Growth Inhibition Assay AND METHODS

Alireza Nematollahi, Noushin Aminimoghadamfarouj, Tracey Bradshaw, Christophe Wiart
2012 unpublished
Since the incidence rate of colon carcinoma is increasing significantly, the present study is undertaken to examine the in vitro cytotoxic activity of two unstudied Malaysian rainforest plants; Uvaria grandiflora Roxb (leaves and stem barks) and Diospyros wallichii King& Gamble (leaves and fruits), on the HTC116 colon carcinoma cell line. Different concentrations of the extracts obtained from each plant were subjected to cytotoxic investigation against HTC116 cell line by using MTT technique.
more » ... ng MTT technique. The chloroform fraction of Uvaria grandiflora stem barks and the chloroform fraction of Diospyros wallichii fruits exhibit the highest growth inhibiting activities among all the tested samples with the GI value 50 of 3.85 µg/ml and 4.58 µg/ml respectively. The obtained results warrant these plants for further bio-assay guided isolation in order to achieve novel leads. ABSTRACT ARTICLE HISTORY In December 2010 Uvaria grandiflora Roxb and Diospyros wallichii King& Gamble were collected from Perak forests in the Ipoh. The plant material was identified by the Forest research institute Malaysia. For each plant sample, plant materials were dried at room temperature in shade and then coarsely powdered. Dried samples were extracted with hexane, chloroform and ethanol by maceration. Dried fractions were obtained after removing the solvent by evaporation under reduced pressure and then were 0 stored at-20 C until tested. colon carcinoma (HCT 116) cell line was sub cultivated twice weekly in RPMI 1640 supplemented with 10% fetal bovine serum and incubated at 37°C in an atmosphere of 95% air, 5% carbon dioxide. To minimize phenotypic drift, cell was maintained in culture for 4 months before being discarded and early passage cells resurrected from liquid nitrogen storage [16]. Cells were seeded into 96-well microtiter plates at a density of 3 5 × 10 per well and were allowed for 24 hours to adhere before drugs were introduced. Serial fractions dilutions were prepared in medium immediately before each assay. Viable cell masses at the 1* 1 2 3
fatcat:um3yqqesfzeqrjxesc7rqq4shm