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ortioetots ofM.Vedicin?e' aod(1 Gen2etics, Stcai/o'rd lU li'ersitv School of.lIe(licitlc, Sta?fo1(rd, Coli/iortn ia 94305 Data are presented indicating that the pSCIO1 plasmid was not derived by recircularization of a mechanically sheared fragment of R6-5 plasmid deoxyribonucleic acid, as was oIriginally believed. During the past several year-s there has been considerable experimental interest in the pSCIO1 plasmid, which has been used as adoi:10.1128/jb.132.2.734-737.1977 fatcat:fqxuptpmtnegznhh5sxgkuihsm