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We optimized a novel nonradioactive hybridization technique using a cis-platin coupled digoxigenin derivative for direct labeling of mRNA. This new mRNA reporter molecule was applied to cDNA membrane arrays to simultaneously identify expression of hundreds of genes. The sensitivity of this nonradioactive mRNA hybridization technique was comparable to radioactive cDNA labeling on housekeeping gene expression but even superior on the detection limit of the expression of low abundant genes usingdoi:10.2144/99275pf01 pmid:10572654 fatcat:s6hw5lpx5ffl7asc5qsu2ag4ju