Cytotoxic Effect of Luteolin on Human Colorectal Cancer Cell Line (HCT-15): Crucial Involvement of Reactive Oxygen Species Brief Communication

Ashok Kumar, Pandurangan, Sudhandiran Ganapasam, Ashok Kumar
2013 Middle East Journal of Cancer   unpublished
Colorectal cancer, a major health concern worldwide, is the third most common form of cancer and second leading cause of cancer-related deaths. The flavonoids are naturally occurring diphenylpropanoids ubiquitous in plant foods and important components of the human diet. Luteolin, a bioflavonoid, possesses many beneficial effects including antioxidant, anti-inflammatory, anti-allergic activities. Methods: We used the HCT-15 colon adenocarcinoma cell line in this study. Cells were treated with
more » ... teolin (100 µM). Results: Membrane damage markers such as alkaline phosphatase and lactate dehydrogenase were analyzed in a time-dependent manner. Luteolin increased reactive oxygen species in a time-dependent manner. DNA damage, a hallmark of apoptosis, was induced by luteolin as analyzed by agarose gel electrophoresis. Conclusion: Luteolin acts as a potential cytotoxic agent that can be used to treat colorectal cancer.