Isolation of Latrodectus Venom and Proteomic Analysis of α-Latrotoxin

2017 International Journal of Research Studies in Biosciences  
Many species of Spiders are venomous organisms, but the venoms of only a few species are wellunderstood, due to the difficulty associated with collecting minute quantities of venom from spiders. Venomous animals use a highly complex cocktails of proteins, peptides and small molecules to subdue and kill their prey. Structure-function studies of spider toxins are leading to the discovery of novel therapeutic routes for neuromuscular diseases, pain and to a variety of other pathological
more » ... ological conditions. α-Latrotoxin is a black widow spider (Latrodectus mactans) venom toxin causing massive neurotransmitter release. This paper presents a protocol for the isolation of venom from Latrodectus mactans using electrical stimulation. The isolated venom is useful for various further proteomic analyses including protein identification, functional assays, and stimulation of venom gene expression for transcriptomic studies. We have done molecular charecterization and modeled α-Latrotoxin structure using in silico Molecular Modeling Technique.
doi:10.20431/2349-0365.0507005 fatcat:vkxdgt6jvbe7rieq6p6sjclxe4