Silicon Nanowires for Intracellular Optical Interrogation with Sub-Cellular Resolution [article]

Menahem Y Rotenberg, Benayahu Elbaz, Vishnu Nair, Erik N Schaumann, Naomi Yamamoto, Laura Matino, Francesca Santoro, Bozhi Tian
2019 bioRxiv   pre-print
Current techniques for intracellular electrical interrogation are substrate−bound and are technically demanding, or lack high spatial resolution. In this work, we use silicon nanowires, which are spontaneously internalized by many cell types, to achieve photo−stimulation with sub−cellular resolution. Myofibroblasts loaded with silicon nanowires remain viable and can undergo cell division. Stimulation of silicon nanowires at separate intracellular locations results in local calcium fluxes. We
more » ... o show that nanowire−containing myofibroblasts can electrically couple to cardiomyocytes in co−culture and that photo−stimulation of the nanowires increases the spontaneous activation rate in neighboring cardiomyocytes. Finally, we demonstrate that this methodology can be extended to the interrogation of signaling in neuron−glia interactions using nanowire−containing oligodendrocytes.
doi:10.1101/825489 fatcat:yktmebdzizdflkzq2woybpv75i