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Objective: Establish whether hemolysis in samples collected from intravenous lines is influenced by catheterization site. Methods: Blood was collected from all patients (67 total) admitted to the emergency department the same morning, through a 20-gauge catheter placed in a vein of the upper limb directly into an evacuated blood tube. Serum was tested for hemolysis index by multi-wavelength photometric readings. Results: The frequency of hemolyzed specimens was 30% (20/67). Hemolysis rate indoi:10.1309/lm2xcv5sqml1ontm pmid:24869000 fatcat:xlac6lpijrfwhipxgok2iu4m54