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The Derivation of Highly Germline-Competent Embryonic Stem Cells Containing NOD-Derived Genome

F. A. Brook, E. P. Evans, C. J. Lord, P. A. Lyons, D. B. Rainbow, S. K. Howlett, L. S. Wicker, J. A. Todd, R. L. Gardner
2003 Diabetes  

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It would be extremely advantageous to the analysis of disease mechanisms in the spontaneous mouse model of type 1 diabetes, the nonobese diabetic (NOD) strain, if genes in this strain could be modified in vivo using embryonic stem (ES) cells and homologous recombination. However, a NOD ES cell line with adequate germline transmission has not yet been reported. We report the development of highly germline-competent ES cell lines from the F1 hybrid of NOD and 129 for use in NOD gene targeting.
more » ... sequently, we developed ES cell lines derived from (NOD ؋ 129)F1 ؋ 129 backcross 1 mice, which were intercrossed to select for homozygosity of particular regions of NOD genome known to contain disease loci.
doi:10.2337/diabetes.52.1.205 pmid:12502514 fatcat:gy4b3su3snd57c5bjoghnka2hm
Citation

F. A. Brook, E. P. Evans, C. J. Lord, P. A. Lyons, D. B. Rainbow, S. K. Howlett, L. S. Wicker, J. A. Todd, R. L. Gardner. "The Derivation of Highly Germline-Competent Embryonic Stem Cells Containing NOD-Derived Genome." Diabetes 52.1 (2003) 205-208