Efficacy of Streptococcus agalactiae vaccine strains N3M and N4M in fry tilapia infected by different strains of S. agalactiae

Sukenda, Sukenda, Arif Lukman Firmansyah, Rahman, Rahman, Sri Nuryati, Dendi Hidayatullah
2018 Jurnal Akuakultur Indonesia  
<p align="center"><strong>ABSTRACT</strong></p><p class="Default"> </p><p>Streptococcus agalactiae is a major bacterial streptococcosis disease that infects tilapia. This study aimed to analyze a specific and nonspecific immune system in fry tilapia that has been given with S. agalactiae vaccine from N<sub>3</sub>M and N<sub>4</sub>M strain and examine the protective immunity against S. agalactiae N<sub>3</sub>M, N<sub>4</sub>M, N<sub>17</sub>O, NK<sub>1, </sub>and N<sub>14</sub>G strains
more » ... ion. Fry tilapia used in this study has the weight of 7.086±0.948 g and length of 7.443±0.353 cm. The S. agalactiae strains that used were N<sub>3</sub>M, N<sub>4</sub>M, N<sub>17</sub>O, NK<sub>1</sub>,and N<sub>14</sub>G. Fry tilapia was vaccinated through intraperitoneal injection method with 0.1 mL per fish of N<sub>3</sub>M and N<sub>4</sub>M vaccines. Fish reared in aquarium sizing of 60×30×50 cm<sup>3</sup> with a density of 10 fishes aquarium<sup>-1</sup>. Two weeks after vaccination, fry tilapia was tested with 0.1 mL fish<sup>-1</sup> of each N<sub>3</sub>M, N<sub>4</sub>M, N<sub>17</sub>O, NK<sub>1</sub>, and N<sub>14</sub>G strains through intraperitoneal injection method. Antibody level measured with indirect enzym-linked immunosorbent assay (ELISA) method. The result of antibody level in vaccinated fish after vaccination of N<sub>3</sub>M, N<sub>4</sub>M vaccine, N<sub>3</sub>M and N<sub>4</sub>M control were 0.767; 0.743; 0.587; and 0.544, respectively. Relative percent survival in N<sub>3</sub>M vaccinated fish after challenged with N<sub>3</sub>M and N<sub>4</sub>M was 87.50% dan 64.70%, respectively, otherwise in N<sub>4</sub>M vaccinated fish was 62.50% dan 76.47%, respectively. N<sub>3</sub>M and N<sub>4</sub>M vaccine strain have better protection as only if it tested with similar bacteria strain.</p><p> </p><p>Keywords: formalin-killed cell, tilapia, protection, Streptococcus agalactiae, strains</p><p><strong> </strong></p><p><strong> </strong></p><p align="center"><strong>ABSTRAK</strong></p><p align="center"><strong> </strong></p><p class="Paragraf">Streptococcus agalactiae merupakan bakteri utama penyakit streptococcosis yang menginfeksi ikan nila. Tujuan penelitian ini adalah menganalisis sistem imun spesifik dan nonspesifik pada benih ikan nila yang diberi vaksin S. agalactiae strain N<sub>3</sub>M dan N<sub>4</sub>M serta mengkaji imunitas protektif terhadap infeksi S. agalactiae strain N<sub>3</sub>M, N<sub>4</sub>M, N<sub>17</sub>O, NK<sub>1</sub>, dan N<sub>14</sub>G. Benih ikan nila yang digunakan memiliki bobot 7,086±0,948 g dan panjang 7,443±0,353 cm. Bakteri yang digunakan adalah S. agalactiae strain N<sub>3</sub>M, N<sub>4</sub>M, N<sub>17</sub>O, NK<sub>1</sub>,danN<sub>14</sub>G. Benih ikan nila divaksinasi menggunakan metode injeksi pada bagian intraperitoneal sebanyak 0,1 mL ekor<sup>-1</sup> vaksin N<sub>3</sub>M dan N<sub>4</sub>M. Pemeliharaan dilakukan pada akuarium berukuran 60×30×50 cm<sup>3</sup> dengan kepadatan 10 ekor akuarium<sup>-1</sup>. Dua minggu setelah vaksinasi benih ikan diuji tantang dengan strain bakteri N<sub>3</sub>M, N<sub>4</sub>M, N<sub>17</sub>O, NK<sub>1</sub>, <sub> </sub>danN<sub>14</sub>G menggunakan metode injeksi pada bagian intraperitoneal dengan dosis 0,1 mL ekor<sup>-1</sup>. Level antibodi diukur dengan metode indirect enzym-linked immunosorbent assay (ELISA). Hasil menunjukkan level antibodi ikan setelah vaksinasi pada perlakuan vaksin N<sub>3</sub>M, N<sub>4</sub>M, kontrol N<sub>3</sub>M, dan kontrol N<sub>4</sub>M berturut-turut 0,767; 0,743; 0,587; dan 0,544. Kelangsungan hidup relatif ikan yang divaksin N<sub>3</sub>M setelah diuji tantang dengan strain N<sub>3</sub>M dan N<sub>4</sub>M berturut-turut 87,50% dan 64,70%, sedangkan pada ikan yang divaksin N<sub>4</sub>M berturut-turut 62,50% dan 76,47%. Vaksin strain N<sub>3</sub>M dan N<sub>4</sub>M memiliki proteksi lebih baik jika diuji tantang dengan strain bakteri yang sama.</p><p class="Default"> </p><p class="Paragraf">Kata kunci: formalin-killed cell, Streptococcus agalactiae, strain, proteksi, nila</p><p> </p><p> </p>
doi:10.19027/jai.17.2.168-180 fatcat:trys34byxrbwpn5riajpgf4oxa