Frequency of Bacille Calmette-Guérin (BCG) andMycobacterium tuberculosisin Tissue Biopsy Specimens of Children Vaccinated With BCG

Maryam Monajemzadeh, Reza Shahsiah, Abdolmajid Zarei, Alireza Alai Alamooti, Fatemeh Mahjoub, Setareh Mamishi, Ghamartaj Khotai, Reza Pazira, Neda Eram
2010 American Journal of Clinical Pathology  
A b s t r a c t Vaccination of all newborns with bacille Calmette-Guérin (BCG) vaccine is a standard practice in developing countries. Disseminated mycobacterial infection in an immunocompromised child can be caused by BCG and other mycobacteria. A total of 21 patients with a histopathologic diagnosis of mycobacterial infection were studied in a period of 4 years. DNA was extracted from formalin-fixed, paraffin-embedded tissues. Real-time polymerase chain reaction was performed to determine the
more » ... ed to determine the mycobacterial species. The overall sensitivity of the assay was 71.5%. The prevalence rates of BCG, Mycobacterium tuberculosis, and other mycobacteria in the positive results were 80% (12/15), 13% (2/15), and 7% (1/15), respectively. From the
doi:10.1309/ajcpxlzprhx9l0yg pmid:20023264 fatcat:roeifrva7vgp5ciqiv24ibz4te