Nasopharyngeal carriage, spa types and drug susceptibility profiles of Staphylococcus aureus from healthy children under 5 years in eastern Uganda [post]

2019 unpublished
Staphylococcus aureus carriage is a known risk factor for staphylococcal disease. However, the carriage rates vary by country, demographic group and profession. This study aimed to determine the S. aureus carriage rate in children in Eastern Uganda, and track Staphylococcus strains that can cause infection in Uganda. Methods: Nasopharyngeal samples from 742 healthy children under 5 years residing in the Iganga/Mayuge Health & Demographic Surveillance Site in Eastern Uganda were processed for
more » ... re processed for isolation of S. aureus. Antibiotic resistance based on minimum inhibitory concentrations (MICs) was determined by the BD Phoenix™ automated identification & susceptibility testing system. Genotyping was performed by spa typing. Results: The processed samples yielded 144 S. aureus isolates (one per child) therefore, the S. aureus carriage rate in children was 19.4% (144/742). Thirty one percent (45/144) of the isolates were methicillin resistant (MRSA) yielding a carriage rate of 6.1% (45/742). All MRSA isolates were susceptible to vancomycin, linezolid and clindamycin however, compared to methicillin susceptible S. aureus (MSSA) (68.8%, 99/144), MRSA isolates were more resistant to non-beta-lactam antimicrobials -tetracycline (91.1%, 41/45), trimethoprim/sulfamethoxazole (73.3%, 33/45), erythromycin (75.6%, 34/45), chloramphenicol (60%, 27/45), gentamicin (55.6%, 25/45) and ciprofloxacin (35.6%, 16/45). Furthermore, one MRSA isolate was mupirocin resistant and 42 (93.3%, 42/45) were multidrug resistant (MDR); three (3%, 3/99)
doi:10.21203/rs.2.10945/v3 fatcat:rvdwtxw6jrdvpnk4satlp3jdyy