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The role of iron in the activation-inactivation of aconitase
1983
Journal of Biological Chemistry
Methods are described for the convenient preparation of aconitase from beef heart mitochondria in its inactive [3Fe-4S] form and largely in its active [4Fe-4S] form. Inactive aconitase can be activated anaerobically by various reducing agents without addition of iron. Under these conditions, maximally 70-80% of the activity attainable in the presence of added iron can be reached. It is concluded that during activation without added iron, [4Fe-4S] clusters are built from [3Fe-4S] clusters at the
pmid:6309829
fatcat:xs2nntlqevfrblmhobnyq46xc4