Journal of Pharmacological Sciences
Proper neuronal morphogenesis is fundamental to the establishment of functional neural circuits. Cyclic adenosine monophosphate (cAMP) has been identified as a key regulator of cellular events among a variety of neurons. Especially, cAMP plays an important role in regulating axonal outgrowth: however, it remains unclear whether and how cAMP regulates axonal branching and elongation independently, because of a lack of techniques that enables spatiotemporal modulation of the intracellular cAMP
... els. Here we utilized photoactivated adenylyl cyclase (PAC), which is originally isolated from Euglena gracilis and produces cAMP either transiently or continuously depending on the exposure time to the blue light. In this talk, we will introduce our application of PAC for the study of axonal morphogenesis, showing that axonal branching and elongation are independently regulated by different signaling pathways. Vascular remodelling is a prominent anatomical feature of CNS disease including multiple sclerosis, stroke, epilepsy, amyotrophic lateral sclerosis, and brain tumour. During development, formation and maturation of vascular network are affected by the factors from neuronal cells. Therefore, we hypothesized that vascular remodelling in pathological CNS may be regulated by neuronal network. In this study, we investigated the role of neuronal change in CNS angiogenesis in the experimental autoimmune encephalomyelitis, an animal model of multiple sclerosis. We used biochemical technique for unveil the key molecule from neuronal cells which activates vascular remodelling. We succeeded the identification of molecule from neuron that promotes vascular endothelial cell proliferation. In this presentation, we will talk our recent data and speculation in involvement of neuronal change in vascular remodelling in CNS disease.