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We have developed multiplexed, high-throughput, quantitative and precise SRM assays for both PTH and VDBP. The MSIA-SRM approach allows rapid and automated enrichment to achieve high sensitivity (ng/L) and selectivity. Simultaneous monitoring of intact and variant PTH species allows the precise quantification of active and inactive forms. Correlation of the PTH MSIA-SRM assay using only the N-terminal peptide (aa 1-13) with the traditional immunoassay confirms that commercial immunoassaysdoi:10.1530/endoabs.32.p156 fatcat:rzlnpuuwcrd77cmmwsgvqijy64