MSIA--SRM assay for parathyroid hormone and vitamin D binding protein: correlation with clinical immunoassay methods and application to clinical samples

Caje Moniz, Lewis Couchman, Bryan Krastins, Mary Lopez, Amol Prakash, David Sarracino, Mary Vogelsang, Scott Peterman, Gouri Vadali, Sarah Robinson
2013 Endocrine Abstracts  
We have developed multiplexed, high-throughput, quantitative and precise SRM assays for both PTH and VDBP. The MSIA-SRM approach allows rapid and automated enrichment to achieve high sensitivity (ng/L) and selectivity. Simultaneous monitoring of intact and variant PTH species allows the precise quantification of active and inactive forms. Correlation of the PTH MSIA-SRM assay using only the N-terminal peptide (aa 1-13) with the traditional immunoassay confirms that commercial immunoassays
more » ... timate the amount of active PTH. Further, no evidence for PTH(7-84) was found in the data set. Further work will validate the utility of VDBP measurement in the routine assessment of vitamin D status.
doi:10.1530/endoabs.32.p156 fatcat:rzlnpuuwcrd77cmmwsgvqijy64