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DNA extraction protocol for low-biomass environmental samples: adapted from the Lucigen MasterPure Complete DNA and RNA Purification Kit manual
[post]
2022
unpublished
Microbial samples collected from built environment surfaces can be very low-biomass. This poses a critical challenge to extract valid signals out of the background noises for downstream analyses. Upon comparing and optimizing several extraction methods, our results demonstrated that bead-beating and heat lysis followed by liquid-liquid extraction was the optimal method, as opposed to widely used column- and magnetic bead-based methods. Adapted from the Lucigen MasterPure Complete DNA and RNA
doi:10.21203/rs.3.pex-1658/v1
fatcat:mgzpupqikvf5hhp6h3gb3lcfou