Rapid micromethod for the purification of Escherichia coli ribonucleic acid polymerase and the preparation of bacterial extracts active in ribonucleic acid synthesis

C Gross, F Engbaek, T Flammang, R Burgess
1976 Journal of Bacteriology  
sine triphosphate [ATP], guanosine triphosphate [GTP], cytidine triphosphate [CTP], and uridine triphosphate [UTP]) were purchased from P-L Biochemicals, and [:3H]UTP (26.9 Ci/mmol) and H235SO4 (NEX-042) were purchased from New England Nuclear. H2355O4 was used without neutralization. Calf thymus DNA and lysozyme (salt free) were purchased from Worthington; tris(hydroxylmethyl)aminomethane (Tris) base, ethylenediaminetetraacetic acid (EDTA), morpholinopro-382 on May 4, 2020 by guest
doi:10.1128/jb.128.1.382-389.1976 fatcat:lp3vowpfojf3tp32i6hfl6d2cm