Investigation of Human Leukocyte Antigen (HLA) Class I and II Alleles and Hepatitis C Virus (HCV) Genotypes in Patients Infected with HCV

Abeer Sharaf, Maha Kawashti, Naglaa Abd El Haliem, Hawazen Ismaeel
2018 unpublished
Hepatitis C virus (HCV) is a major public health problem with more than 130-180 million people infected worldwide. Several studies in different populations have reported the association of human leukocyte antigen (HLA) genotypes, and HCV viral load, and genotypes. The aim of this study was to investigate a possible association between HLA class I and II alleles in HCV-infected patients and healthy individuals. This study was conducted on 82 individuals characterized as group A: 29 healthy
more » ... A: 29 healthy screened blood donors as controls; group B: 7 patients positive for HCV antibodies with non-detectable HCV-RNA; and group C: 46 patients positive for HCV antibodies with detectable HCV-RNA. HCV seropositivity was determined by enzyme immuno-assay (EIA), confirmed by recombinant immune blotting assay (RIBA). Viral RNA was detected by qualitative polymerase chain reaction (PCR) followed by determination of viral load by quantitative RT-PCR. HCV DNA amplicons were utilized by immune blotting hybridization assay for detection of HCV genotypes in HCV-infected groups.