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Chromatography is a technique which is used for the separation of constituents in a mixture. This technique involves 2 phases stationary and mobile phases. separation of this constituents is based on the difference between partition coefficients of the two phases. High Performance Liquid Chromatography is analytical technique for separation, dentification and quantification of constituents in mixture. HPLC is an advanced technique of column liquid chromatography. UPLC – Ultra performance liquiddoi:10.5281/zenodo.1401200 fatcat:xfgivn534ng4fclumfbvb7f5zm