Hu Koyunlarında MBL Gen İntronlarının Polimorfizmi ve MBL Serum Seviyeleri ile İlişkisi
Polymorphisms of MBL Gene Introns and Their Association with MBL Serum Levels in Hu Sheep

Mengting ZHAI, Jian MOU, Mengting ZHU, Yanping LIANG, Mingyuan WANG, Zongsheng ZHAO, Hongmei ZHANG
2019 Kafkas Universitesi Veteriner Fakultesi Dergisi  
PCR single-strand conformation polymorphism (SSCP) and DNA sequencing techniques were used to analyze the genetic polymorphism of mannose-binding lectin (MBL) gene 3 introns in Hu sheep. The results showed that 3 introns of MBL gene had polymorphism, 3 genotypes were identified in intron1, which were controlled by 2 alleles, respectively; 3 genotypes were identified in intron2, which were controlled by 2 alleles, respectively; 3 genotypes were identified in intron3, which were controlled by 2
more » ... e controlled by 2 alleles, respectively; 6 new single nucleotide polymorphisms(SNPs) mutation sites were found, g.288T>A in intron1, g. 1091 T>C, g.1096A>C, g.1770G>C in intron 2, g.2297C>T, g.2331G>A in intron3. Use ELISA to detect MBL serum level in Hu sheep, then use One-way ANOVA analyze the relationship of different genotypes and MBL serum level. And the result showed that the MBL serum level of AA genotype was higher than BB genotype (P<0.05) in intron1, CC genotype was higher than DD, GG genotype was higher than HH (P<0.05) in intron2. So, AA, CC and GG genotypes may be related to disease resistance; BB, DD and HH genotypes be related to disease susceptibility. The point mutation in intron3 couldn't lead to the change of MBL serum level.
doi:10.9775/kvfd.2018.20969 fatcat:nilshspl5nfqfpondy3ihauphm