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Vibrio parahaemolyticus (V. parahaemolyticus) is the commonest source of seafood poisoning and has a very high incidence in the countries of Asian. Aims and Objectives: This study aims to investigate the presence of V. parahaemolyticus in seafood from Brunei seafood market using the Most Probable Number-Polymerase Chain Reaction (MPN-PCR). Results: None of the twnety-three seafood samples that were purchased at random from unselectively chosen wet markets and hypermarkets in Brunei Darussalamdoi:10.3126/ajms.v5i2.8948 fatcat:hbuz7sc4nvbydfl4qx2br7j36a