Downloaded from FIGURE 4. Expression and antimicrobial activity of the mBD14-Ig fusion protein. a, schematic drawing of the mBD14-Ig fusion protein. b, Western blot analysis of the recombinant expressed mBD14-Ig fusion protein using an peroxidase-conjugated donkey anti-human IgG monoclonal antibody for detection. MW, molecular weight. c, antimicrobial assays were performed as described under "Experimental Procedures" using the indicated strains of E. coli and S. aureus. To determine the number

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... f colony-forming units, serial dilutions were plated with or without the indicated concentrations of mBD14-Ig fusion protein, and colony counts were performed 24 h later. Data are means Ϯ S.D. of one representative experiment of three, each done in triplicate. Mouse ␤-Defensin 14 (mBD14), the Orthologue of hBD3 5418 JOURNAL OF BIOLOGICAL CHEMISTRY VOLUME 283 • NUMBER 9 • FEBRUARY 29, 2008 by guest on July 25, 2018 http://www.jbc.org/ Downloaded from FIGURE 5. Chemotactic activity of mBD14 and hBD3. The migration of HEK 293 cell stably expressing the chemokine receptor mouse CCR6 (a) and freshly isolated mouse resident peritoneal cells (b) in response to various concentrations of recombinant expressed mBD14-Ig and hBD3-Ig was tested using a 48-well chemotaxis chamber assay as described under "Experimental Procedures." Data are means Ϯ S.D. of one representative experiment of three, each done in triplicate.