Singh-Mustapha Melt Curve

Prashant Singh
2017 Figshare  
Real-time polymerase chain reaction (PCR) multiplex assays are a convenient method for detection of multiple target sequences in one reaction. Multiplexing based on melt curve analysis further offers an inexpensive yet accurate method for detection of multiple targets. Standardization of a multiplex melting curve assay with an internal amplification control can be a complicated and time-consuming process. Here, we compiled the most important factors that should be taken into consideration in
more » ... consideration in the standardization of a melting curve real-time PCR assay.
doi:10.6084/m9.figshare.5493169.v1 fatcat:t3bgijd3p5bfpcmhcnettjiape