Green synthesis of AgNPs@PPE and its Pseudomonas aeruginosa biofilm formation activity compared to pomegranate peel extract
International Journal of Nanomedicine
Bacteria are able to form biofilm on the biotic and abiotic surfaces which helps to protect themselves from deleterious conditions, predation, desiccation, and exposure to antibacterial substances. About 80% of bacterial infections are caused by those bacteria living in the biofilm. Pseudomonas aeruginosa, a gram-negative, non-fermentative bacillus, and the ubiquitous bacterium is an important opportunistic pathogen notorious for biofilm formation and is remarkably resistant against most
... tics multiple front-line antibiotics, which significantly contributes to eradication failure. The aim of this paper was to evaluate the anti-biofilm formation activity of Ag@PPEs gainst P. aeruginosa bacteria. An aqueous extract of black pomegranate peel was used for the synthesis of silver nanoparticles (AgNPs@PPE). The characteristics, anti-biofilm formation and cell toxicity of AgNPs@PPE were examined in vitro. Absorbance at λmax 372 nm which is related to the surface plasmon resonance, confirms the AgNPs@PPE formation. XRD pattern showed the face-centered qubic (fcc) crystalline structure of AgNPs. TEM images showed that spherical AgNPs size is ranged between 32 and 85 nm. The AgNPs@PPE showed inhibition effect against P. aeruginosa biofilm formation at 0.1 to 0.5 mg/ml concentrations. Cell toxicity assay showed that at 400 µg/ml, AgNPs@PPE were safe without a significant toxicity in L929 cell line. These data indicate that co-treatment of PPE and AgNPs@PPE significantly decreased the biofilm formation rate. Furthermore, no significant toxicity of AgNPs@PPE was shown against L929 cell line at 400 µg/ml concentration.