Polyamine Limitation of Growth Slows the Rate of Polypeptide Chain Elongation in Escherichia coli

Caroline M. Jorstad, David R. Morris
1974 Journal of Bacteriology  
The rate of polypeptide chain elongation during steady-state, polyaminelimited growth of a mutant of Escherichia coli was measured by two independent techniques. Analysis of polysome patterns gave values of 17.5 and 9.5 amino acids per s at 37 C in unstarved and polyamine-limited cells, respectively. From the kinetics of entry of labeled amino acids into polypeptides of defined molecular weights, values at 30 C of 10.1 and 5.8 amino acids per s were obtained for unstarved and polyamine-limited
more » ... polyamine-limited cultures, respectively. Correction of these values to 37 C resulted in rates of 15.0 and 8.7 amino acids per s. These results support the previous conclusion, based on the kinetics of ,B-galactosidase induction, that polyamine starvation decreases the rate of protein synthesis by limiting the velocity of polypeptide chain elongation.
doi:10.1128/jb.119.3.857-860.1974 fatcat:b4gc6bkonreotljsp5iwad2pg4